Generator

Part:BBa_K1312000:Design

Designed by: Tatsui yusuke   Group: iGEM14_Kyoto   (2014-09-30)


generate some magnetosome proteins


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 8
    Illegal NheI site found at 31
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1608
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1073
    Illegal NgoMIV site found at 1399
    Illegal NgoMIV site found at 2007
    Illegal AgeI site found at 1665
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 271


Design Notes

original sequence of mamB has PstI's recognition site so we pointed mutation


Source

This part come from magnetotactic bacteria AMB-1's genomic sequence.

References